Laboratory Equipment
The Edinburgh Cell Wall Group is equipped with:
- two Dionex HPLCs
- two scintillation counters
- a radioisotope thin-layer analyser (AR-2000 Bioscan Imaging scanner),
- a high-voltage (5 kV) electrophoresis system (with tanks set up at pH 2.0, 3.5, and 6.5),
- a multilabel plate reader (Victor X3 PE 2030; measuring luminiscence, fluorescence, UV/VIS absorbance),
- two digital imaging systems (MultiDoc-It UV/VIS for TLC, paper-chromatogram and paper-electrophoretogram size),
- Rotofor liquid-phase IEF purification system (BioRad) + HV PowerPac Power Supply (BioRad) + Desktop unichiller (Huber)
- BlueHorizon IEF electrophoresis system (Serva)
- UV-visible spectrophotometer
- speedVac concentrators and freeze dryer,
- benchtop and high-speed chilling centrifuges,
- ultracentrifuge,
- benchtop shakers (microtitration plate format & bigger formats)
- block heaters (for MT plates, 1-2 ml, 15 ml and 50 ml test tubes)
- three balances (± 1 mg and ±10 µg),
- tissue culture facilities,
- full-size paper chromatography tanks,
- IBM-compatible computers, printers and A3 scanner,
- several fraction collectors,
- sonicators,
- ultraturrax,
- fan ovens (+260 °C; +310 °C)
- and all the routine equipment needed for the work.
Other facilities available within the Institute of Molecular Plant Sciences include fluorescence microscopy, electron microscopy, automated autoradiogram developing and a mass-spectrometry service.
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High voltage paper electrophoresis tanks with buffers at pH 2.0, 3.5, and 6.5. |
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UV imaging system (254 and 366 nm UV, and white light) for paper-chromatograms and paper-electrophoretograms. |
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Bioscan radioactivity detector |
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Victor X3 Multilabel plate reader |
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BioRad Rotofor purification system |
Services to other laboratories
The Edinburgh Cell Wall Group performs carbohydrate analyses using the Dionex HPLC.
It also performs preparative and analytical work using high-voltage paper electrophoresis
and paper chromatography. Non-labelled and labelled substrates for carbohydrate and
phenolic work can be supplied on a commercial or collaborative basis. A fluorescent
'dot-blot' assay kit for xyloglucan endotransglycosylase (XET) and mixed-linkage glucan
: xyloglucan endotransglycosylase (MXE) activity testing can be supplied as well. For
details, please contact Prof. Fry.