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Separations

Flow Imaging in Separation Capillaries

David Simpson, Sandy Yates, George Alder, John Knox and Pat Langridge-Smith

The small column volumes found in capillary electrophoresis and capillary electrochromatography are usually advantageous. However when capillaries are jointed together using conventional HPLC fittings, dead volumes (which are small compared to the volumes of HPLC columns) become a problem. We have begun to investigate methods for producing joints suited to the volumes involved. Initial work has focused on using computational fluid dynamics to evaluate jointing strategies, and in using laser induced fluorescence to monitor the progression of bands through capillary columns. A paper was recently submitted for the Desty Memorial Lecture for Innovation in Separation Science, held at the Royal Institution of Great Britain on the 18th October 1999, which won joint third prize.

Desty Memorial Lecture Paper