methods

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Plasmids
competent cells (Robb)
plasmid miniprep (Robb)


 Proteins
GST-fusion protein (Robb)
Protein purification using the Bac system (Helen)
Western blotting (Eilidh)
Non-denaturing PAGE (Eilidh)
MBD refolding (Helle)
Protein / DNA interaction
GST pulldown (Robb)
ChIP method (Robb)
ChIP method (Helen)
Bandshift method (Helen)

DNA analysis
Bisulfite sequencing (Jennifer)
Direct sequencing from colony (Jennifer)
Methylating DNA with Sss1 methylase [Jennifer]
LOH analysis (Jennifer)
Glycosylase assay (Eilidh)
Annealing oligonucleotides (Eilidh)

RNA analysis
RNA FISH method 1 (Helen)
RNA FISH method 2 (Helen)


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